Jul. 28, 2026
Determining the Required UV Dose for Inactivation of Various Microorganisms using 280nm LEDsJoint Research Conducted by Nichia and Tokushima University
Tokushima, Japan – 28 July 2026: Nichia, the world's largest LED/Laser Diode manufacturer and inventor of the high-brightness blue and white LEDs, has established a joint research course in the field of microbial control at the Graduate School of Biomedical Sciences, Tokushima University (Professor Akira Takahashi, Department of Preventive Environment and Nutrition), and has conducted evaluations of ultraviolet-induced inactivation of various microorganisms, including fungi, bacteria, and viruses. Through this joint research, the UV dose required for the inactivation of various microorganisms using 280nm LEDs, the peak wavelength of Nichia's mass-produced UV-C LEDs, was determined. The results are presented below.
Inactivation of microorganisms using UV light has been widely studied with mercury lamps, tunable wavelength lasers, and LEDs. In recent years, UV LEDs have been gaining attention as a solution for infection control equipment, due to the development of various wavelengths and the small form factor providing design flexibility. However, evaluation systems and measurement methods suitable for accurately assessing UV sensitivity with UV LEDs have not yet been fully established. Irradiation conditions vary amongst studies, making it difficult to directly compare and evaluate microbial inactivation performance across different research results. To address these challenges, Nichia recognized the need for a standardized irradiation device and evaluation method and developed a UV LED irradiation device in 2023 based on strictly controlled irradiation conditions.
For more information about this device, refer to the link below.
Nichia Developed a UV Irradiant Device to Evaluate UV Sensitivity
Since 2023, Nichia and Tokushima University have conducted joint research using this device to evaluate the effectiveness of different UV wavelengths for inactivating various microorganisms, including fungi, bacteria, and viruses. Following the publication of a paper summarizing the research results, Nichia has compiled the UV dose required for the inactivation of various microorganisms using 280nm LEDs, which correspond to the peak wavelength of Nichia's mass-produced UV-C LEDs, while considering not only the inactivation effectiveness but practical considerations such as the LED output and lifetime characteristics. The results for the fungi (6 species), bacteria (11 species), and viruses (12 species) are shown below.
| Microbial Category |
Common Name |
Scientific Name | UV Dose(mJ/cm2)at Each Inactivation Rate |
||
|---|---|---|---|---|---|
| 90% | 99% | 99.9% | |||
| Fungi | Yeast | Rhodotorula mucilaginosa NBRC114835 | 63 | 84 | 100 |
| Mold | Cladosporium Halotolerans NBRC113481 | 820 | 1450 | 2250 | |
| Cladosporium sphaerospermum NBRC6348 | 57 | 88 | 149 | ||
| Penicillium roqueforti NBRC5459 | 37 | 61 | 105 | ||
| Aspergillus brasiliensis NBRC9455 | 98 | 180 | 400 | ||
| Botrytis cinerea Persoon ATCC46522 | 104 | 128 | 187 | ||
| Bacteria | Bacteria | Escherichia coli ATCC25922 | 4.5 | 6.4 | 7.7 |
| Staphylococcus aureus ATCC29213 | 4.2 | 5.4 | 6.6 | ||
| Vibrio parahaemolyticus RIMD2210633 | 1.2 | 2.5 | 3.8 | ||
| Enterococcus faecalis ATCC7080 | 7.5 | 10 | 11.4 | ||
| Pseudomonas aeruginosa PAO-1 strain | 1.8 | 3 | 4.4 | ||
| Salmonella enterica Enteritidis 171 strain | 3 | 4.8 | 6.5 | ||
| Legionella pneumophila ATCC33152 | 3 | 4.5 | 6.2 | ||
| Campylobacter jejuni NCTC11168 | 2.1 | 3.7 | 5.2 | ||
| Lactobacillus plantarum ATCC8014 | 5.7 | 8.1 | 10.1 | ||
| Bacillus subtilis IFO3134 (trophozoite) | 4.5 | 6.7 | 8.3 | ||
| Bacillus subtilis IFO3134 (spore) | 36 | 48 | 61 | ||
| Viruses | Virus | Influenza A virus H1N1 subtype PR8 strain | 2 | 5 | 9.4 |
| Herpes simplex virus KOS strain | 5 | 12 | 19 | ||
| Human coronavirus OC43 strain | 2.7 | 5.2 | 7.7 | ||
| Human coronavirus 229E strain | 3 | 5 | 9.7 | ||
| Feline calicivirus F9 strain | 8.8 | 20.6 | 30 | ||
| Human adenovirus 5 strain | 30 | 56 | - | ||
| Human respiratory syncytial virus strain Ⅼong | 1.6 | 2.6 | 3.9 | ||
| Human respiratory syncytial virus strain 18537 | 1.7 | 3.1 | 4.6 | ||
| Influenza A virus H5N1 subtype | 3.5 | 7.7 | 12.6 | ||
| Human metapneumovirus strain TN/83-1211 | 3.8 | 5.4 | 7.1 | ||
|
Severe acute respiratory syndrome coronavirus 2
|
2.5 | 5.4 | 7.6 | ||
| Escherichia coli phage MS2 NBRC102619 | 23.9 | 48.2 | - | ||
For detailed information on the test conditions and evaluation results, refer to the link below.
UV-C LEDs for Disinfection UV
These research findings are expected to help improve the accuracy of UV LED-based microorganism inactivation evaluations and support the establishment of appropriate test conditions. By continuing to utilize this device, Nichia will advance the selection of optimal LEDs, equipment design, and irradiation conditions based on standardized and comparable evaluations across different wavelengths, contributing to the wider practical adoption and commercialization of UV-based technologies.
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